Would non-host resistance in barley prove a source of durable, race-nonspecific resistance for wheat?
Researcher / PhD Candidate : Mathilde Daniau
Mathilde’s project consists in identifying and characterising barley genes involved in non-host resisance to leaf rust and powdery mildew both in barley and in wheat.
Nonhost resistance (NHR), defined as the immunity displayed by all genotypes of a plant species towards all pathotypes of a potential pathogenic species, is the most common form of disease resistance in plants. It may be based on Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) and Effector-Triggered Immunity (ETI).
This durable immunity of plant genotypes to a broad-spectrum of potential pathogens suggests that NHR is highly relevant for agricultural applications. However, its genetic basis still remains unclear.
The current research project aims to a better understanding of NHR in barley through two complementary approaches:
– One, based on forward genetics, involves the fine mapping of a 80 cM QTL in barley which has shown to confer resistance to several heterologous rusts including the wheat leaf rust Puccinia triticina;
– The other one, built on reverse genetics, aims to functionally characterize two barley Receptor-Like Kinases (RLKs) genes which have shown, from previous research at IPK (Gatersleben, DE) using transient system, to play a role in NHR to the wheat powdery mildew (Blumeria graminis tritici – Bgt). The current project aims to validate this by stable over-expression studies. The transfer of these genes into wheat and the assessment of the resulting wheat transgenic lines for response to Bgt inoculation might determine whether genes involved in NHR in barley can confer host resistance when transferred to wheat.
These two approaches will lead to identify new components involved in basal resistance and to better understand nonhost resistance. This knowledge may be applied in breeding programs for resistance.
Aspects of Mathilde’s PhD research form part of a bigger, ERA-CAP project: DURESTrit (Nov. 2014-Oct. 2017), co-ordinated by Dr Patrick Schweizer, IPK, Gatersleben, Germany. Consequently Mathilde has been included in this European collaboration.
Wageningen University: Dr. Ir. Rients E. Niks
NIAB: Dr. Lesley A. Boyd
MATHILDE’S EDUCATIONAL BACKGROUND
- 2014-2018 : PhD candidate at the Plant Breeding laboratory of Wageningen University and Research Center (NL), in partnership with the department of Genetics and Pre-Breeding at the National Institute of Agricultural Botany (NIAB, UK)
- 2011-2014 : Master’s degree in agronomy from the ISA (Lille, FR) http://www.isa-lille.com/
- 2009-2011 : Bachelor’s degree in biology and biotechnology from the Catholic University of Lille, France http://www.univ-catholille.fr/about-us/welcome.asp