Numerous dominant spring or recessive winter alleles at the vernalization locus VRN-H1 have been previously described. Within the European context, these were systematically investigated (PMID 17713756), identifying seven spring and two winter alleles. Spring Vrn-H1 alleles are differentiated from winter alleles, predominantly due to the presence of deletions within intron 1. Combined haplotypes fromKASPar markers HvVRNH1_SNP2 and HvVRNH1_0P5_InDel (

see reference allele sequence table below for haplotype combinations from these two KASPar markers) can be used to identify all but one of the known VRN-H1 alleles (allele 1B, PMID 17713756. Note: allele 1B is relatively rare in European barley).

The HvVRNH1_0P5_InDel KASPar assay is a pseudo-SNP, assaying for the presence or absence of the Insertion. When the insertion is absent (scored in the genotype data below as '0'), the assay returns a SNP call of 'G'. All other calls represent instances when the insertion is present (score '1' below).

Note: when no DNA is present in a well, a call of '?' will be made. Thus, final genotype call for a given accession using this assay should be based on more than one repetition of the assay.

PMID: 21115826; 17713756