NIAB - National Institute of Agricultural Botany

Assay information: HvVRS1_C349G

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Narrative

Three independent loss-of-function mutations (vrs1.a1, vrs1.a2, vrs1.a3) within the HvHOX1 gene underlying the VRS1 genetic locus result in the conversion of the dominant wild-type 2-row phenotype to the recesive 6-row phenotype. This marker (HvVRS1_C349G) assays for the SNP underlying the vrs1.a3 6-row allele. SNP C = 2-row (trait score 1). SNP G = 6-row (trait score 2).

Note: see position 1020bp in reference sequence alignment.

Note: Genotypes for all three mutations need to considered together for identification of all instances of the 6-row phenotype. KASPar marker HvVRS1_GINS681 assays for the SNP underlying the vrs1.a1 allele. The assay for the 'T' insertion/deletion characeristic of 6-row allele vrs1.a2 (PMID: 17220272) failed to convert to the KASPar platform.

References

PMID: 17220272

Map AGOUEB ver1
Chromosome 2H
cM position 85.92
Assay ID 9720125
Assay Name HvVRS1_C349G
Reference Allele Sequences
Sequence ID Allele Phenotype
gi|224487838|gb|AB489123 C 2-row (cv Pamella Blue, allele Vrs1.b2)
gi|224487834|gb|AB489121 C 2-row (cv Bonus, allele Vrs1.b3)
gi|224487836|gb|AB489122 G 6-row (cv Natsudaikon Mugi allele Vrs1.a3)
Polymorphism sequence TCTGGAGCTGAGCTT[C/G]CGGGAGGACCGCAAG
Reference allele sequence alignment
Jalview displaying file: HvVRS1_C349G_reference.aln
Validation plot
Genotype data
Accession Name Other Ref Genotype call (with filter) Reference sample of known genotype
Maris_Otter AFP 2/232 C
Golden_Promise AFP 2/235 C
Triumph AFP 2/278 C
Golf AFP 2/421 C
Klaxon AFP 2/456 C
Halycon AFP 2/514 C
Pipkin AFP 2/578 C
Dandy AFP 2/674 C
Blenheim AFP 2/688 C
Tyne AFP 2/750 C
Puffin AFP 2/806 C
Pastoral AFP 2/816 C
Posaune AFP 2/820 C
Gypsy AFP 2/884 C
Clarine AFP 2/899 C
Alexis AFP 2/904 C
Fighter AFP 2/947 C
Manitou AFP 2/951 G
Sprite AFP 2/954 C
Bronze AFP 2/994 C
Blanche AFP 2/1002 C
Derkado AFP 2/1011 C
Dallas AFP 2/1022 C
Graphic AFP 2/1029 C
Chariot AFP 2/1031 C
Willow AFP 2/1055 C
Swift AFP 2/1058 C
Intro AFP 2/1071 C
Felicie AFP 2/1091 C
Linnet AFP 2/1127 C
Delibes AFP 2/1133 C
Cooper AFP 2/1146 C
Brewster AFP 2/1147 C
Melanie AFP 2/1160 U
Sunrise AFP 2/1162 C
Rhythm AFP 2/1169 C
Tempo AFP 2/1170 C
Fanfare AFP 2/1171 C
Vivaldi AFP 2/1173 C
Emeraude AFP 2/1183 C
Hanna AFP 2/1184 C
Cork AFP 2/1187 C
Optic AFP 2/1188 C
Brahms AFP 2/1191 C
Canasta AFP 2/1194 C
Reggae AFP 2/1196 C
Riviera AFP 2/1203 C
Tokyo AFP 2/1210 C
Arctic AFP 2/1211 C
Muscat AFP 2/1214 G
Mystique AFP 2/1219 C
Prelude AFP 2/1221 C
Crown AFP 2/1230 G
Tankard AFP 2/1238 C
Wren AFP 2/1242 C
Ragtime AFP 2/1244 C
Clarity AFP 2/1246 C
Trinidad AFP 2/1256 C
Volley AFP 2/1262 C
Rifle AFP 2/1263 C
Cobalt AFP 2/1264 C
Tiffany AFP 2/1266 C
Antigua AFP 2/1276 C
Emilia AFP 2/1281 C
Spice AFP 2/1285 C
Quartet AFP 2/1291 C
Landlord AFP 2/1293 C
Rainbow AFP 2/1301 C
Charm AFP 2/1303 C
Torup AFP 2/1311 C
Pearl AFP 2/1318 C
Jewel AFP 2/1320 C
Baton AFP 2/1326 C
Spirit AFP 2/1334 C
Candy AFP 2/1345 C
Ricarda AFP 2/1350 C
Bistro AFP 2/1392 C
Angela AFP 2/1397 G
Heligan AFP 2/1402 C
Tabetha AFP 2/1404 C
Acorn AFP 2/1409 C
Century AFP 2/1410 C
Decanter AFP 2/1411 C
Linden AFP 2/1415 C
Acapella AFP 2/1419 C
Horizon AFP 2/1423 C
Static AFP 2/1427 C
Pongo AFP 2/1431 C
Opal AFP 2/1435 C
Wizard AFP 2/1442 C
Steeple AFP 2/1443 C
Arrow AFP 2/1445 C
Rounder AFP 2/1457 C
Siberia AFP 2/1463 C
Halifax AFP 2/1466 C
Artist AFP 2/1468 C
Leonie AFP 2/1471 C
Berwick AFP 2/1474 C
Otira AFP 2/1485 C
Potter AFP 2/1492 C
Saloon AFP 2/1501 C
Anvil AFP 2/1511 C
Haka AFP 2/1528 C
Sarah AFP 2/1534 C
Carola AFP 2/1535 C
Violet AFP 2/1540 C
Akita AFP 2/1555 C
Prestige AFP 2/1566 C
Prot AFP 2/1567 C
County AFP 2/1572 C
Pewter AFP 2/1575 C
Brise AFP 2/1576 C
Viskosa AFP 2/1579 C
Neruda AFP 2/1580 C
Agenda AFP 2/1589 C
Azure AFP 2/1591 C
Alabama AFP 2/1597 C
Pippa AFP 2/1599 C
Diamond AFP 2/1604 C
Carat AFP 2/1606 C
Tucker AFP 2/1608 C
Pict AFP 2/1626 G
Scylla AFP 2/1628 C
Spire AFP 2/1668 C
Spike AFP 2/1669 C
Sunbeam AFP 2/1697 C
Cannock AFP 2/1700 C
Kestrel AFP 2/1705 C
Sequel AFP 2/1717 G
Outlook AFP 2/1726 C
Mead AFP 2/1728 C
Rakaia AFP 2/1741 C
Pelican AFP 2/1745 C
Catalina AFP 2/1746 C
Cocktail AFP 2/1769 C
Brazil AFP 2/1780 C
Camion AFP 2/1825 C
Mandolin AFP 2/1841 C
Carafe AFP 2/1862 C
Toby AFP 2/1864 C
Doyen AFP 2/1865 C
Drum AFP 2/1867 C
Rebecca AFP 2/1871 C
Saffron AFP 2/1880 C
Amarena AFP 2/1886 C
Faraday AFP 2/1892 C
Balasun AFP 2/1902 C
Thalia AFP 2/1919 C
Concept AFP 2/1923 C
Westminster AFP 2/1939 C
Oxford AFP 2/1940 C
SW Macsena AFP 2/1951 C
Waggon AFP 2/1963 C
NFC Tipple AFP 2/1966 C
Timori AFP 2/1974 C
Colibri AFP 2/2003 C
Corsica AFP 2/2025 C
Rummy AFP 2/2034 C
Silicon AFP 2/2040 C
Tuscon AFP 2/2050 C
Centurion AFP 2/2054 C
Paramount AFP 2/2055 C
Accent AFP 2/2073 C
Pelican AFP 2/2077 C
Acrobat AFP 2/2102 C
Calico AFP 2/2104 C
Quench AFP 2/2121 C
Azalea AFP 2/2180 C
Tamise AFP 2/2188 C