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Molecular Characterisation of Disease resistance in OSR


Contact: Dr David Kenyon
                Dr Jane Thomas


Working on the two principle pathogens of oilseed rape, Leptosphaeria maculans and Pyrenopeziza brassicae, quantitative real-time PCR has been utilised to map the development of the pathogens in host tissue over thermal time. In the case of P. lingam this relates to the growth of hyphae down the petiole to the stembase whereas with P. brassicae it is accumulation of biomass in leaf tissue.  Critical time points have been identified and used to describe a cultivars resistance response  stem canker and light leaf spot.

         

Leading on from previous studies in this area on the major pathogens of OSR there is a need to validate the methodologies developed prior to there adoption for use in policy support studies for Defra and commercial trials on behalf of plant breeders.

These techniques also for the basis of our involvement in the OSR genetic improvement network.

The financial support of DEFRA and CEL (HGCA) is gratefully acknowledged.