NIAB - National Institute of Agricultural Botany
Plant Science Into Practice
Dr David Lee
Tel: +44 (0)1223 342481/342484
Fax: +44 (0)1223 277602
E-mail: Dr David Lee
Research Interests
Development of DNA molecular markers, their use in genetic analyses for variety identification, food provenance and traceability. PI for Food Standards Agency-funded collaborative project with Central Science Laboratory (York) investigating an alternative method (QUIZ) for quantifying GMO content in food samples.
Current Projects
• BBSRC cis-acting regulatory elements in the rice genome: a novel source of alleles for rice breeding
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• Eurigen: European Rice Genetic Diversity
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• Food Standards Agency QUIZ: Quantitation using Informative Zeros
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• CO-EXTRA: GM and non-GM supply chains: their CO-EXistence and TRAceability.
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Completed Projects
• DNA-TRACK: Traceability of DNA fragments throughout the food chain by DNA/PNA technologies. Application to novel foods. QLK1-CT-2000-01658.
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• OLIV-TRACK: Traceability of origin and authenticity of olive oil by combined genomic and metabolomic approaches. QLK1-CT-2002-02386.
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• Food Standards Agency: Traceability of origin and authenticity of olive oil by combined genomic and metabolomic approaches. Q01091.
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• Development of Molecular Markers for DUS Testing in Oilseed Rape.
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Recent Publications
Pasquali M, Komjati H, Lee D and Bayles R (2010). SRAP technique efficiently generates polymorphisms in Puccinia striiformis isolates. Journal of Phytopathology (in press).
La Mura M, Norris C, Sporle S, Jayaweera D, Greenland A and Lee D (2010). Development of 5S DNA genome-specific markers in Brassicas and related species for hybrid testing. Genome (in press).
Gruden K, Allnutt TR, Ayadi M, Baeumler S, Bahrdt C, Berben G, Berdal KG, Bertheau Y, Andersen CB, Brodmann P, Gašparič MB, Burns MJ, Burrel AM, Cankar K, Esteve T, Holst-Jensen A, Kristoffersen AB, La Paz J, Lee D, Løvseth A, MacArthur R, Morisset D, Pla M, Rud RB, Skjæret C; Tengs T, Valdivia H, Wulff D, Zhang D, Žel J (2010) Reliability and cost of GMO detection. In: GM and Non-GM Food Supply Chains: Co-Existence and Traceability, Chapter 5. Eds. Davison J and Bertheau Y, Blackwell Publishers.
Lee D, La Mura M, Allnutt T, Powell W and Greenland A (2009). Isothermal amplification of GM DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping. Journal of Agricultural and Food Chemistry (in press), DOI: 10.1021/jf902455j.
Kalendar R, Lee D and Schulman AH (2009). FastPCR software for PCR primer and probe design and repeat search. Genes, Genomes and Genomics (in press).
Lee D, Lupotto E and Powell W (2009). G-string slippage turns white rice red. Genome (in press).
Lee D, La Mura M, Allnutt TR and Powell W (2009). Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences. BMC Biotechnology 9:7, doi:10.1186/1472-6750-9-7.
Lee D, La Mura M, Greenland A and Mackay I (2008). Quantitation using informative zeros (QUIZ): application for GMO detection and quantification without recourse to certified reference material. Food Chemistry, doi:10.1016/j.foodchem.2008.05.118.
Doveri S, Sabino GF, Díaz A, Reale S, Busconi M, da Câmara Machado A, Martín A, Fogher C, Donini P and Lee D (2008). The use of a set of standardised microsatellite markers for cultivar identification and genetic relationship studies in olive (Olea europaea L.). Scientia Horticulturae 116: 367–373.
Doveri S and Lee D (2007). Development of sensitive crop-specific PCR assays using 5S DNA and their application for testing DNA extraction protocols.
Journal of Agricultural and Food Chemistry 55: 4640-4644.
Consolandi C, Palmieri L, Doveri S, Maestri E, Marmiroli N, Reale S, Lee D, Baldoni L, De Bellis G and Castiglion B (2007). Olive variety identification by ligation detection reaction (LDR) of alleles in a universal array format.
Journal of Biotechnology 129: 565-574.
Doveri S, O’Sullivan DM and Lee D (2006). Non-concordance between genetic profiles of olive oil and fruit: a cautionary note to the use of DNA markers for provenance testing.
Journal of Agricultural and Food Chemistry 54: 9221 -9226.
Reale S, Doveri S, Díaz A, Angiolillo A, Lucentini L, Pilla F, Martín A, Donini P and Lee D (2006). SNP-based markers for discriminating olive (Olea europaea L.) cultivars.
Genome 49: 1193-1205.
Martellossi C, Taylor E, Lee D, Graziosi G and Donini P (2006). DNA extraction and analysis from processed coffee beans.
Journal of Agricultural and Food Chemistry 53: 8432 –8436.
Vaseva-Gemisheva I, Lee D, Alexieva V and Karanov E (2005). Response of Pisum sativum cytokinin oxidase/dehydrogenase expression and specific activity to drought stress and herbicide treatments.
Plant Growth Regulation 46: 199-208.
Acquadro A, Lee D, Donini P, Portis E and Lanteri S (2005). Development and characterization of microsatellite markers in Cynara cardunculus L.
Genome 48: 217-225.
Taylor EJA, Konstantinova P, Leigh F, Bates JA and Lee D (2004). Gypsy-like retrotransposons in Pyrenophora species: an abundant and informative class of molecular markers.
Genome 47: 519-525.
Chiapparino E, Lee D and Donini P (2004). Genotyping single nucleotide polymorphisms (SNPs) in barley by tetra-primer ARMS-PCR.
Genome 47: 414-420.
Bardini M, Lee D, Donini P, Mariani A, Gianì S, Toschi M, Lowe C and Breviario D (2004). TBP: a new tool for testing genetic diversity in plant species based on functionally relevant sequences.
Genome 47: 281-291.
Vaseva-Gemisheva I, Lee D, Alexieva V and Karanov E (2004). Cytokinin oxidase/dehydrogenase in Pisum sativum plants during vegetative development. Influence of UV-B irradiation and high temperature on enzymatic activity.
Plant Growth Regulation 42: 1-5.
Acquadro A, Lee D, Chiapparino E, Comino C, Portis E, Donini P and Lanteri S (2003).
Detection and characterisation of SNPs in Capsicum spp. Capsicum and Eggplant Newsletter 22: 37-40.
Leigh F, Kalendar R, Lea V, Lee D, Donini P and Schulman AH (2003). Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques.
Molecular General Genomics 269: 464-474.
Uzun B, Lee D, Donini P and Çağırgan Mİ (2002). Identification of a molecular marker linked to the closed capsule mutant trait in sesame (Sesamum indicum L.) using AFLP.
Plant Breeding 122: 95-97.
Tommasini L, Batley J, Arnold GM, Cooke RJ, Donini P, Lee D, Law JR, Lowe C, Moule C, Trick M and Edwards KJ (2002). The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties.
Theoretical and Applied Genetics 106: 1091-1101.
Taylor EJA, Stevens EA, Bates JA, Morreale G, Lee D, Kenyon DM and Thomas JE (2001). Rapid-cycle PCR detection of Pyrenophora graminea from barley seed.
Plant Pathology 50: 347-355.
Corbett G, Lee D, Donini P and Cooke RJ (2001). Identification of potato varieties by DNA profiling.
Acta Horticulturae 546: 387-390.
Others
• Jaccoud-Filho DS, Lee D, Reeves JC and Yorinori JT (1996). Characterisation of a seed-borne fungal pathogen Phomopsis phaseoli f. sp. meridionalis: the agent of soya bean stem canker.
In Seed Health Testing: Progress Towards the 21st Century pp 147-152 Eds JD Hutchins and JC Reeves. CAB International, Oxon.
• Lee D and Cooke RJ (2006). Molecular Markers for DUS Testing in Oilseed Rape.
Plant It! Issue 9.
• Doveri S, Lee D, Maheswaran M and Powell W (2007). Molecular Markers for Mapping and Breeding.
In, Principles & Practices of Plant Molecular Mapping & Breeding, Chapter 2, Ed C Kole.