1.1 Selection of Diploid and Tetraploid Donor Lines
• 50 Ae. tauschii selected from collection of 430 accessions.
• 3 T. durum identified as tetraploid parent.
1.2 Generation of New SHW and Crossing with Hexaploid Bread Wheat
• 15 D genome donors captured in novel SHW
• 61 F1 created capturing all available SHW with Paragon or Robigus
• 33 SHW backcrossed to Paragon or Robigus
• SSD underway from BC1F2 of 9 BC1F1
1. 3 Crossing Hexaploid Bread Wheat with Tetraploid Emmer and Durum Donor Lines
• Methodology developed for TxHW
• F1s created capturing 29 domesticated tetraploids (15 in both Paragon and Robigus) and 27 wild emmers (14 in both Paragon and Robigus)
• BC1F2 created for 3 combinations
• 10 domesticated emmers, 34 wild emmers and 2 durum wheats evaluated for photosynthetic efficiency traits
1.4 Field Plots and Line Progression
• Autumn sown tussock trials in 2010-2011 and 2011-2012.
• Both provided excellent evaluation of morphological traits and disease resistance in tetraploid accessions.
• Germination and establishment of Ae. tauschii was very poor in both years and little data was generated.
• 13 different F1s produced capturing maximum diversity from 161 T. dicoccoides accessions studied by NIAB and JIC
• F2 seed produced from each F1 cross
• 4 crosses to be taken forwards through SSD
3.1. D genome donor
• Backcrossing SHW containing initial D genome donor JIC2220007 to paragon aborted because of low SNP polymorphisms between 2 parents
• SHW with 4 different D genomes evaluated by UoB to identify maximum D genome diversity
• Backcrossing between 2 SHW and Paragon now underway.
3.2 AB donor
• Ttd140 crossed to Paragon.
• 140 BC1 created. Further F1s will be backcrossed to augment number of BC1 available.
• Suite of evenly distributed SNPs identified by UoB for evaluating genome coverage.
• BAC library completed and publically available.
Paper will be published in TAG; A strategy for exploiting exotic germplasm using genetic, morphological and environmental diversity: the Aegilops tauschii Coss. example.’